Protein Assays

Protein Assays Below is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/ touchstone of take enquireed, subjective comments on accuracy and convenience, and major interfering agents. procedural details, equipment requirements, and references are outlined in the individual assay documents. The criteria for option of a protein assay are usually based on convenience, availability of protein for assay, presence or absence of interfering agents, and fill for accuracy. For example, the Lowry method is very sensitive just is a cardinal step procedure that requires a minimum of 40 proceedings incubation time. The Bradford assay is more sensitive and can be read within 5 minutes, however proteins with low arginine instruct will be depreciated. globally, estimates are more accurate for tortuous mixtures of proteins. Estimates of concentration of pure proteins can be very rough depending on the principle of the assay, unless the same pure protein is used as a standard. Criteria will be discussed in the individual documents.

Because incompatible proteins have different amino acid compositions, the sensitivity of colorimetrical assays to individual proteins may vary widely. The most reprodicible results are obtained with standards key out up of a mixture of proteins that is as similar as realistic to the unknown. For most purposes, a relative amount is pricey enough, but the standard used should be reported. For example, the Bradford assay is practically more sensitive to bovine serum albumin (BSA) than to immune gamma globulin G (IgG), so that with ! IgG the investigator is likely to overrate the amount of protein in a sample. With BSA the investigator is likely to underestimate the amount. General Reference: Stoscheck, CM. Quantitation of Protein. Methods in Enzymology 182: 50-69 (1990).If you want to get a broad essay, order it on our website: OrderCustomPaper.com

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